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SOLVED: A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the Tm (ºC), which is computed by this equation 81.5 + 0.41 (%
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Solved Melting temperature (Tm) is the temperature at which | Chegg.com
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Tm Calculator
Vintage First Calculator TM-25 Cell Electronic Small Pocket Calculator Auto Off | eBay